Effect of several electrolyzed waters on the skin permeation of lidocaine, benzoic Acid, and isosorbide mononitrate

Effect of several electrolyzed waters on the skin permeation of lidocaine, benzoic Acid, and isosorbide mononitrate

Kitamura T, Todo H, Sugibayashi K.
Faculty of Pharmaceutical Sciences, Josai University, Saitama, Japan

Abstract

The effects of several electrolyzed waters were evaluated on the permeation of model base, acid and non-ionized compounds, lidocaine (LC), benzoic acid (BA), and isosorbide mononitrate (ISMN), respectively, through excised hairless rat skin. Strong alkaline-electrolyzed reducing water (ERW) enhanced and suppressed the skin permeation of LC and BA, respectively, and it also increased the skin permeation of ISMN, a non-ionized compound. On the contrary, strong acidic electrolyzed oxidizing water (EOW) enhanced BA permeation, whereas suppressing LC permeation. Only a marginal effect was observed on the skin permeation of ISMN by EOW. These marked enhancing effects of ERW on the skin permeation of LC and ISMN were explained by pH partition hypothesis as well as a decrease in skin impedance. The present results strongly support that electrolyzed waters, ERW and EOW, can be used as a new vehicle in topical pharmaceuticals or cosmetics to modify the skin permeation of drugs without severe skin damage.

PMID: 19065312 [PubMed – indexed for MEDLINE]

Editor’s note: Strong ionized alkaline water with a pH of 10+ is great for de-greasing, stain removal and external temporary pain relief. For skin care, we typically recommend ionized acidic water with a pH of 5-5.5 for its antibacterial and skin-softening effects and because it is the same pH of the skin. See the full list of uses for ionized water at different pH levels here.

Disclaimer: The testimonials, videos, articles and comments herein are the expressed opinions and experiences of the individual persons shared under their 1st amendment right of free speech. The information on this website has not been reviewed by the FDA. Products offered for sale herein are not intended to treat, cure or prevent any disease or health condition. No medical claims are being made. Click here to see why we must say that.

 

Antimicrobial effects and efficacy on habitually hand-washing of strong acidic electrolyzed water–a comparative study of alcoholic antiseptics and soap and tap water

Antimicrobial effects and efficacy on habitually hand-washing of strong acidic electrolyzed water–a comparative study of alcoholic antiseptics and soap and tap water

Sakashita M, Iwasawa A, Nakamura Y.
Department of Nursing, Showa University Fujigaoka Hospital.

The rate of bacterial elimination for the stamp method was compared with regular hand-washing (using soap and tap water), hygienic hand-washing (using alcoholic antiseptics), and hand-washing using strong acidic electrolyzed water (the SAEW method) in routine work. After routine work, the average number of bacteria remaining on the nurse’s hands with using the SAEW-method, rubbing method and tap water method, were: 54 +/- 63, 89 +/- 190, 128 +/- 194 CFU/agar plate, respectively (n = 81). In this study. It was clarified that a much larger number of Bacillus sp. were detected for the rubbing method than for the other methods. After further nurse work, the most number of absorbed bacteria on a nurse’s hands were counted after cleaning a patient’s body. The rate of bacteria elimination for hand-washing with soap and tap water after taking care of a patient was insufficient, especially when before care was provided the number of bacteria on the nurse’s hands were less than 100 CFU/agar plate. From these results, the following manual for sanitary hand washing is recommended: 1. At first, dirty hands should be cleaned and the number of bacteria should be reduced using soap and tap water or by scrubbing with disinfectants. 2. After the number of bacteria has been reduced, use the SAEW method routinely. 3. For care requiring a high level of cleanliness or if no tap water facilities are available, use the rubbing method. Finally, routine use of the SAEW method in ICU could be recommended with conventional disinfectants and soap and tap water on a case by case basis for less than adverse reactions, such as in the case of rough-hands or keeping a low level of bacteria on hands.

Editor’s note: Not only is ionized acidic water from a Chanson water ionizer a wonderful hand washing solution for killing bacteria, but it is great for all over use on skin and hair due to being closer to the body’s natural pH.

Disclaimer: The testimonials, videos, articles and comments herein are the expressed opinions and experiences of the individual persons shared under their 1st amendment right of free speech. The information on this website has not been reviewed by the FDA. Products offered for sale herein are not intended to treat, cure or prevent any disease or health condition. No medical claims are being made. Click here to see why we must say that.

 

Grape Test

Grape Test

Hydration matters. Healthy human adult bodies are, by volume, around 70% water. The brain is around 90% water. Less water than this means loss of full physical and mental potential. In this simple test, three grapes were immersed in different containers for 60 seconds each.

  • The first grape was placed in a jar containing alkaline water.
  • The second grape was placed in a jar containing acid water.
  • The third grape in the test was placed in a jar containing ordinary tap water.

The grapes were then left in the air at room temperature and photographed over the course of the following ten weeks.

grape test that shows alkaline water giving more hydration

The testimonials, videos, articles and comments herein are the expressed opinions and experiences of the individual persons shared under their 1st amendment right of free speech. The information on this we site has not been reviewed by the FDA. Products offered for sale herein are not intended to treat, cure or prevent any disease or health condition. No medical claims are being made.

 

Disclaimer: The testimonials, videos, articles and comments herein are the expressed opinions and experiences of the individual persons shared under their 1st amendment right of free speech. The information on this website has not been reviewed by the FDA. Products offered for sale herein are not intended to treat, cure or prevent any disease or health condition. No medical claims are being made. Click here to see why we must say that.

 

Steel Nail Test

Steel Nail Test

In the simple test below, each jar contains two ordinary steel nails. Ionized alkaline water was added to the jar on the left. Ordinary tap water was added to the jar on the right. Ionized alkaline water quickly showed antioxidant benefits, while the tap water quickly showed oxidizing, or accelerated aging.

steel nail test that shows alkaline water slowing down the rusting

Takeaway: By providing your body with adequate amounts of fresh, filtered ionized alkaline water, you may slow natural signs of aging through a significant intake of antioxidants. Shop for a Chanson water ionizer now!

 

Disclaimer: The testimonials, videos, articles and comments herein are the expressed opinions and experiences of the individual persons shared under their 1st amendment right of free speech. The information on this website has not been reviewed by the FDA. Products offered for sale herein are not intended to treat, cure or prevent any disease or health condition. No medical claims are being made. Click here to see why we must say that.

 

Reduction of Staphylococcus aureus in atopic skin lesions with acid electrolytic water–a new therapeutic strategy for atopic dermatitis

Reduction of Staphylococcus aureus in atopic skin lesions with acid electrolytic water–a new therapeutic strategy for atopic dermatitis

Sasai-Takedatsu M, Kojima T, Yamamoto A, Hattori K, Yoshijima S, Taniuchi S, Namura S, Akamatsu H, Horio T, Kobayashi Y.

Department of Pediatrics, Kansai Medical University, Osaka, Japan.

The subjects studied were 22 pediatric patients newly diagnosed with atopic dermatitis (AD); 11 were treated with acid electrolytic water (AEW), which has a strong bactericidal activity (AEW group), and the other 11 with tap water (placebo group). AEW or tap water, 1 ml/cm2 (body surface area), was sprayed on their skin lesions with a spray gun each twice a day for a week. There were no significant differences between the two groups in regard to sex, age, serum IgE, peripheral eosinophil counts, grading scores of AD, and duration of AD. The study was designed as a randomized, placebo-controlled, double-blind clinical trial. Colony counts of Staphylococcus aureus on skin lesions in the AEW group, both 3 min after spraying (P < 0.05) and after 1 week of skin treatment (P < 0.01), were significantly decreased as compared with colony counts before treatment, while there was no significant difference in the placebo group before and after treatment. Grading scores of AD also decreased in the AEW group (P < 0.01), but not in the placebo group. Both the subjects “guardians” evaluation and a referee physician’s evaluation of treatment effect were significantly higher in the AEW group than in the placebo group (P < 0.01). AEW may be potentially effective in preventing a staphylococcal chronic inflammation in AD because of its strong bactericidal activity.

Disclaimer: The testimonials, videos, articles and comments herein are the expressed opinions and experiences of the individual persons shared under their 1st amendment right of free speech. The information on this website has not been reviewed by the FDA. Products offered for sale herein are not intended to treat, cure or prevent any disease or health condition. No medical claims are being made. Click here to see why we must say that.

 

The bactericidal effects of electrolyzed oxidizing water on bacterial strains involved in hospital infections

The bactericidal effects of electrolyzed oxidizing water on bacterial strains involved in hospital infections

Vorobjeva NV , Vorobjeva LI, Khodjaev EY.
Artificial Organs 2004 Jun;28(6):590-2.
Department of Physiology of Microorganisms, Biology Faculty, Moscow State University, Lenin Hills 1/12, Moscow 119992, Russia. nvvorobjeva@mail.ru

The study is designed to investigate bactericidal actions of electrolyzed oxidizing water on hospital infections. Ten of the most common opportunistic pathogens are used for this study. Cultures are inoculated in 4.5 mL of electrolyzed oxidizing (EO) water or 4.5 mL of sterile deionized water (control), and incubated for 0, 0.5, and 5 min at room temperature. At the exposure time of 30 s the EO water completely inactivates all of the bacterial strains, with the exception of vegetative cells and spores of bacilli which need 5 min to be killed. The results indicate that electrolyzed oxidizing water may be a useful disinfectant for hospital infections, but its clinical application has still to be evaluated.

PMID: 15153153 [PubMed – in process]

Disclaimer: The testimonials, videos, articles and comments herein are the expressed opinions and experiences of the individual persons shared under their 1st amendment right of free speech. The information on this website has not been reviewed by the FDA. Products offered for sale herein are not intended to treat, cure or prevent any disease or health condition. No medical claims are being made. Click here to see why we must say that.

 

Acidic or Hyper-oxidized Water in Dentistry

Acidic or Hyper-oxidized Water in Dentistry

Sterilization of Dental Equipment to Prevent Infections.

Transcript from TV presentation at Jikei Dental Clinic, Tokyo.

Announcer A: “It`s time for our Special Report. They say that in our mouths there are about 80 kinds of bacteria. When your teeth are treated, your blood vessels and gums can be damaged, raising the danger of infection from the hepatitis virus.”

Announcer B: “The key to preventing such infection is sterilization of the dental equipment. The power of disinfectant water created by electrolysis is now being studied.”

Reporter: “You are watching various kinds of bacteria through a microscope. Are these bacteria from ditch water, or from someone`s kitchen? Neither. They are in fact bacteria from the mouth that live in small cracks between our teeth. In our mouth, there are about eighty kinds of bacteria, including Tricomonas candida and MRSA against which even antibiotics do not work any more.”

Dr. Kashiwada: “This is a heated air turbine. It sounds like this and water comes out from here.”

[on screen] Jikei Dental Clinic, Tokyo

Reporter: “When you have an intense toothache, the place you run to is the dentist`s office. There, without exception, your will be taken care of by this air turbine. It rotates 500,000 times a minute with compressed air, scraping away at your tooth. When the instrument is employed, water is used to prevent vibration and friction from heat. The trouble is, when the turbine is stopped, it sucks in water.”

Dr. Kashiwada: “Many kinds of turbines with an attachment to prevent this reverse flow have been brought out on the market lately. When a turbine is stopped, water gets sucked back in along with blood and other things from the patient`s mouth.”

Reporter: “Are AIDS or hepatitis viruses hiding in this blood?”

Dentist: “It can be very dangerous. So, dentists disinfect the turbine after every use.”

Reporter: “Disinfecting a turbine takes time, money and causes wear on the instrument. There is yet another problem in handling an air turbine.”

Dentist: “When water is splashed into the patient`s mouth, water splashes out.”

Reporter: “We experimented with water using red dye to see how far the water splashed.”

Dentist: “The water that splashes off the teeth is mixed with bacteria, blood and saliva. So, it becomes very unsanitary.”

Reporter: “There are many bacteria living in our mouths. Not only do the germs splash all over the dental equipment, endangering other patients, but they can infect the staff as well.”

Reporter: “The staff could develop an infection, huh?”

Dentist: “Yes, we must be careful.”

Reporter: “This office has a side vacuum system now to prevent the splashing of water. It`s good equipment, but it is not yet in common use.”

Dr. Kashiwada: “In dentistry, we deal with many kinds of bacteria and it has been very difficult to kill them all. But, now we can use a superbly effective disinfectant called hyperoxidized water.”

Reporter: “This is the equipment that creates the hyperoxidized water. By adding a small amount of salt to tap water and then applying electrolysis through a water ionizer, a powerfully disinfectant water is created.”

Dr, Kashiwada: “”Hyperoxidized water has been studied at universities and research institutes. We gathered the available information and created this chart.”

Reporter: “Crosses indicate no disinfecting ability, triangles indicate not effective all the time, and circle indicate effectiveness.”

Dr. Kashiwada: “The results show that the hyperoxidized water is very effective in eradicating viruses such as AIDS and hepatitis B. It can be said that strong hyperoxidized water has the same or better disinfectant effectiveness as conventional drugs.”

Reporter: “Dr. Kashiwada uses the disinfectant properties of this water to sterilize the air turbine.”

Dr. Kashiwada: “The air turbine becomes filthy after use. First, we wipe it off with a towel that is soaked with strong hyperoxidized water. Then the turbine is turned on to push out unclean water from inside. Right before the turbine stops, it is put into hyperoxidized water. In this way, hyperoxidized water is sucked back into the tube, disinfecting the inside of the turbine.”

Reporter: “After that, air is bubbled out from the turbine, and finally it is wiped again by a sterilized towel.”

Reporter: “Research is being conducted to confirm the effectiveness of hyperoxidized water in preventing in-clinic infections caused by splashing when using an air turbine. The amazing thing is that some dentists now have abandoned conventional sterilization equipment that use ultra sonic waves, heat or gas.”

Dr. Kashiwada: “Conventional sterilization has never been perfect. The disinfectant effects of strong hyperoxidized water shortens sterilization time drastically, and we can be sure of its effectiveness. It makes me wonder if it could be an epoch-making method.”

Reporter: “There is more to this.”

Dr. Kashiwada: “We no longer use any conventional sterilization here. Everything has been replaced by this water.”

Reporter: “When administering Novocane, the tooth is now splashed with hyperoxidized water. It should be noted that cleaning the inside of the mouth involves dousing it with plenty of hyperoxidized water, then all the bacteria die just like this.” [on screen]

Dr. Okamura: “We have just extracted a wisdom tooth at the back of the jaw. Can you see that there is very little bleeding?”

Reporter: “Dr. Okamura started to pay attention to hyperoxidized water three years ago. He purchased a small unit and is using the hyperoxidized water regularly.”

Dr. Okamura: “We have found that it is very effective in accelerating the healing of the surgical wound and in arresting the bleeding. It makes it easier to perform surgery.”

Reporter: “It was surprising to see that they were using an IV bag for the hyperoxidized water. It is easier to carry them around in this way. The hospital is happy not to have to invest in more equipment.”

Reporter: “What happens if you accidentally drink this hyperoxidized water? Doesn`t it cause any problem if this water gets into the eyes? We flew to Kyushu to visit the Chemical Inspection Association which tested the toxicity of this hyperoxidized water.”

Mr. Imatanaka: “To see if it was toxic when accidentally ingested, we fist conducted acute toxicity tests. Absolutely no toxicity was detected. Then we test it on a rabbit to see if contact with the eye would cause irritability. Nothing unusual was seen in the rabbit`s eye.”

Reporter: “Powerful disinfectant, hyperoxidized water — it is non-toxic and can suppress bleeding. It is expected that from now on it will be indispensable in dentistry.”

Disclaimer: The testimonials, videos, articles and comments herein are the expressed opinions and experiences of the individual persons shared under their 1st amendment right of free speech. The information on this website has not been reviewed by the FDA. Products offered for sale herein are not intended to treat, cure or prevent any disease or health condition. No medical claims are being made. Click here to see why we must say that.

 

Antibacterial effect of electrolyzed water on oral bacteria

Antibacterial effect of electrolyzed water on oral bacteria

Lee SH, Choi BK.

Department of Oral Microbiology and Immunology, School of Dentistry, Seoul National University, Seoul, Republic of Korea.

Abstract

This study investigated the antibacterial effect of electrolyzed water on oral bacteria both in vitro and in vivo. Tap water was electrolyzed in a water vessel using platinum cell technology. The electrolyzed tap water (called Puri-water) was put in contact with five major periodontopathogens or toothbrushes contaminated with these bacteria for 30 sec. In addition, Puri-water was used as a mouthwash for 30 sec in 16 subjects and the antibacterial effect on salivary bacteria was evaluated. Puri-water significantly reduced the growth of all periodontopathogens in culture and on toothbrushes, and that of aerobic and anaerobic bacteria in saliva, when compared to the effect of tap water. It also significantly reduced mutans streptococci growing on mitis salivarius-bacitracin agar. Our results demonstrate that the electrolyzed tap water is effective as a mouthwash and for toothbrush disinfection.

PMID: 16953177 [PubMed – indexed for MEDLINE]
Dent Mater J. 2001 Jun;20(2):148-55.

Editor’s note: “Electolyzed tap water” is another name for ionized water. In this case, they are using ionized acidic water to kill bacteria in the form of mouthwash and toothbrush sterilization. We use Chason ionized acidic water to do both on a regular basis! Think how much money you would save if you never had to buy another bottle of mouthwash again! With a Chanson water ionizer, you won’t have to! And oral health care is only one of the many uses of Chanson ionized acidic water, see the more complete list here.

Disclaimer: The testimonials, videos, articles and comments herein are the expressed opinions and experiences of the individual persons shared under their 1st amendment right of free speech. The information on this website has not been reviewed by the FDA. Products offered for sale herein are not intended to treat, cure or prevent any disease or health condition. No medical claims are being made. Click here to see why we must say that.

 

Efficiency of electrolyzed oxidizing water on reducing Listeria monocytogenes contamination on seafood processing gloves

Efficiency of electrolyzed oxidizing water on reducing Listeria monocytogenes contamination on seafood processing gloves

Liu C, Su YC.
College of Food Science and Technology, Shanghai Fisheries University, 334 Jungong Road, Shanghai 200090, PR China.

Food processing gloves are typically used to prevent cross-contamination during food preparation. However, gloves can be contaminated with microorganisms and become a source of contamination. This study investigated the survival of Listeria monocytogenes on gloves and determined the efficacy of electrolyzed oxidizing (EO) water for reducing L. monocytogenes contamination on seafood processing gloves. Three types of reusable gloves (natural rubber latex, natural latex, and nitrile) and two types of disposable gloves (latex and nitrile) were cut into small pieces (4 x 4 cm(2)) and inoculated with 5-strain L. monocytogenes cocktail (5.1 x 10(7) CFU/cm(2)) with and without shrimp meat residue attached to surfaces. L. monocytogenes did not survive well on clean reusable gloves and its populations decreased rapidly to non-detectable levels within 30 min at room temperature. However, high levels of Listeria cells were recovered from clean disposable gloves after 30 min of inoculation. Presence of shrimp meat residue on gloves enhanced the survival of L. monocytogenes. Cells of L. monocytogenes were detected on both reusable and disposal gloves even after 2 h at room temperature. Soaking inoculated gloves in EO water at room temperature for 5 min completely eliminated L. monocytogenes on clean gloves (>4.46 log CFU/cm(2) reductions) and significantly (p < 0.05) reduced the contamination on soil-containing gloves when compared with tap water treatment. EO water could be used as a sanitizer to reduce L. monocytogenes contamination on gloves and reduce the possibility of transferring L. monocytogenes from gloves to RTE seafoods.

Editor’s note: Electrolyzed oxidizing (EO) water is another name for ionized acidic water. We use and recommend ionized acidic water from a Chanson water ionizer as an all-natural green cleaning alternative to harsh chemicals that can be used extensively for kitchen sterilization.

Disclaimer: The testimonials, videos, articles and comments herein are the expressed opinions and experiences of the individual persons shared under their 1st amendment right of free speech. The information on this website has not been reviewed by the FDA. Products offered for sale herein are not intended to treat, cure or prevent any disease or health condition. No medical claims are being made. Click here to see why we must say that.

 

Inactivation of Escherichia coli O157:H7, Salmonella enteritidis and Listeria monocytogenes on the surface of tomatoes by neutral electrolyzed water

Inactivation of Escherichia coli O157:H7, Salmonella enteritidis and Listeria monocytogenes on the surface of tomatoes by neutral electrolyzed water

AIMS:

To determine the efficacy of neutral electrolyzed water (NEW) in killing Escherichia coli O157:H7, Salmonella enteritidis and Listeria monocytogenes, as well as nonpathogenic E. coli, on the surface of tomatoes, and to evaluate the effect of rinsing with NEW on the organoleptic characteristics of the tomatoes.

METHODS AND RESULTS:

The bactericidal activity of NEW, containing 444 or 89 mg l(-1) of active chlorine, was evaluated over pure cultures (8.5 log CFU ml(-1)) of the above-mentioned strains. All of them were reduced by more than 6 log CFU ml(-1) within 5 min of exposure to NEW. Fresh tomatoes were surface-inoculated with the same strains, and rinsed in NEW (89 mg l(-1) of active chlorine) or in deionized sterile water (control), for 30 or 60 s. In the NEW treatments, independent of the strain and of the treatment time, an initial surface population of about 5 log CFU sq.cm(-1) was reduced to <1 log CFU sq.cm(-1), and no cells were detected in the washing solution by plating procedure. A sensory evaluation was conducted to ascertain possible alterations in organoleptic qualities, yielding no significant differences with regard to untreated tomatoes.

SIGNIFICANCE AND IMPACT OF THE STUDY:

Rinsing in NEW reveals as an effective method to control the presence of E. coli O157:H7, S. enteritidis and L. monocytogenes on the surface of fresh tomatoes, without affecting their organoleptic characteristics. This indicates its potential application for the decontamination of fresh produce surfaces.

Editor’s note: Ionized acidic water from a Chanson water ionizer is an all-natural chemical-free sterlizating agent that can be used on produce. We recommend a spray or quick dip in the ionized acidic water and then a ten minute soak in ionized alkaline water shortly before consuming to revive the produce to its natural state.

Disclaimer: The testimonials, videos, articles and comments herein are the expressed opinions and experiences of the individual persons shared under their 1st amendment right of free speech. The information on this website has not been reviewed by the FDA. Products offered for sale herein are not intended to treat, cure or prevent any disease or health condition. No medical claims are being made. Click here to see why we must say that.